ISO 7954:1987Withdrawn

Microbiology — General guidance for enumeration of yeasts and moulds — Colony count technique at 25 degrees C

Microbiologie — Directives générales pour le dénombrement des levures et moisissures — Technique par comptage des colonies à 25 degrés C

This edition has been replaced by ISO 21527-1:2008, ISO 21527-2:2008.

Abstract / scope

The method consists in preparing of poured plates using a specified selective culture medium and a specified quantity of a test sample, further dilutions of the test sample. Then aerobic incubation of the plates at 25 degrees centigrade for 3, 4 or 5 days, followed by calculation of the number of yeasts and moulds per gram or per millilitre of the sample from the number of colonies obtained on plates chosen at dilution levels so as to give a significant result.

Life cycle

Replaced by

ISO 21527-1:2008PublishedMicrobiology of food and animal feeding stuffs — Horizontal method for the enumeration of yeasts and moulds — Part 1: Colony count technique in products with water activity greater than 0,95International Standard · Published 1 Jul 2008 · ISO/TC 34/SC 9 ISO 21527-2:2008PublishedMicrobiology of food and animal feeding stuffs — Horizontal method for the enumeration of yeasts and moulds — Part 2: Colony count technique in products with water activity less than or equal to 0,95International Standard · Published 1 Jul 2008 · ISO/TC 34/SC 9