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ISO 21528-1:2004Withdrawn

Microbiology of food and animal feeding stuffs — Horizontal methods for the detection and enumeration of Enterobacteriaceae — Part 1: Detection and enumeration by MPN technique with pre-enrichment

Microbiologie des aliments — Méthodes horizontales pour la recherche et le dénombrement des Enterobacteriaceae — Partie 1: Recherche et dénombrement à l'aide de la technique NPP avec préenrichissement

This edition has been replaced by ISO 21528-1:2017.

Abstract / scope

ISO 21528-1:2004 specifies a method, with pre-enrichment, for the detection of Enterobacteriaceae. It is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

Enumeration is carried out by calculation of the most probable number (MPN) after incubation at 37 °C in liquid medium.

This method is applicable when the microorganisms sought are expected to need resuscitation before enrichment, and when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.

A limitation on the applicability of ISO 21528-1:2004 is imposed by the susceptibility of the method to a large degree of variability

Life cycle

Replaces

ISO 8523:1991WithdrawnMicrobiology — General guidance for the detection of Enterobacteriaceae with pre-enrichmentInternational Standard · Published 3 Oct 1991 · ISO/TC 34/SC 9ISO 7402:1993WithdrawnMicrobiology — General guidance for the enumeration of Enterobacteriaceae without resuscitation — MPN technique and colony-count techniqueInternational Standard · Published 16 Sep 1993 · ISO/TC 34/SC 9ISO 5552:1997WithdrawnMeat and meat products — Detection and enumeration of Enterobacteriaceae without resuscitation — MPN technique and colony-count techniqueInternational Standard · Published 4 Dec 1997 · ISO/TC 34/SC 6

Replaced by

ISO 21528-1:2017PublishedMicrobiology of the food chain — Horizontal method for the detection and enumeration of Enterobacteriaceae — Part 1: Detection of EnterobacteriaceaeInternational Standard · Published 16 Jun 2017 · ISO/TC 34/SC 9