ISO 4831:2006Published

Microbiology of food and animal feeding stuffs — Horizontal method for the detection and enumeration of coliforms — Most probable number technique

Microbiologie des aliments — Méthode horizontale pour la recherche et le dénombrement des coliformes — Technique du nombre le plus probable

Abstract / scope

ISO 4831:2006 gives general guidelines for the detection and the enumeration of coliforms. It is applicable to products intended for human consumption and for the feeding of animals, and to environmental samples in the area of food production and food handling.

Enumeration is carried out by calculation of the most probable number (MPN) after incubation in a liquid medium at 30 °C or 37 °C.

This enumeration method is applicable when the number sought is expected to be in the range 1 to 100 per millilitre or per gram of test sample.

Life cycle

Replaces

ISO 3811:1979WithdrawnMeat and meat products — Detection and enumeration of presumptive coliform bacteria and presumptive Escherichia coli — (Reference method)International Standard · Published 1 Nov 1979 · ISO/TC 34/SC 6 ISO 5541-2:1986WithdrawnMilk and milk products — Enumeration of coliforms — Part 2: Most probable number technique at 30 degrees CInternational Standard · Published 13 Nov 1986 · ISO/TC 34/SC 5 ISO 4831:1991WithdrawnMicrobiology — General guidance for the enumeration of coliforms — Most probable number techniqueInternational Standard · Published 14 Mar 1991 · ISO/TC 34/SC 9