ISO 20837:2006Withdrawn

Microbiology of food and animal feeding stuffs — Polymerase chain reaction (PCR) for the detection of food-borne pathogens — Requirements for sample preparation for qualitative detection

Microbiologie des aliments — Réaction de polymérisation en chaîne (PCR) pour la détection des micro-organismes pathogènes dans les aliments — Exigences relatives à la préparation des échantillons pour la détection qualitative

This edition has been replaced by ISO 22174:2024.

Abstract / scope

ISO 20837:2006 provides criteria and examples for sample preparation in order to obtain PCR-compatible samples or nucleic acids of suitable quality and quantity for PCR.

It provides a description of the general principles involved. References to standards concerning the enrichment of microorganisms are given in Annex A, and a detailed method for DNA extraction is given in Annex B.

ISO 20837:2006 has been established for food matrices, but could also be applied to feed and agricultural/environmental matrices with some adaptations, if necessary.

Life cycle

Replaced by

ISO 22174:2024PublishedMicrobiology of the food chain — Polymerase chain reaction (PCR) for the detection and quantification of microorganisms — General requirements and definitionsInternational Standard · Published 16 Aug 2024 · ISO/TC 34/SC 9