ISO/TS 16649-3:2005Withdrawn

Microbiology of food and animal feeding stuffs — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli — Part 3: Most probable number technique using 5-bromo-4-chloro-3-indolyl-beta-D-glucuronide

Microbiologie des aliments — Méthode horizontale pour le dénombrement des Escherichia coli beta-glucuronidase positive — Partie 3: Technique du nombre le plus probable utilisant le bromo-5-chloro-4-indolyl-3 beta-D-glucuronate

This edition has been replaced by ISO 16649-3:2015.

Abstract / scope

ISO/TS 16649-3:2005 specifies a horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli, by means of the liquid-medium culture technique and calculation of the most probable number (MPN) after incubation at 37 °C, then at 44 °C.

This Technical Specification is applicable to products intended for human consumption and the feeding of animals, and environmental samples in the area of food production and food handling.

The method is suitable for the enumeration of cells of Escherichia coli that may have been subjected to stress arising from dehydration, freezing, exposure to a saline (such as marine) environment or damage by disinfectants such as chlorine-containing products.

Life cycle

Replaced by

ISO 16649-3:2015PublishedMicrobiology of the food chain — Horizontal method for the enumeration of beta-glucuronidase-positive Escherichia coli — Part 3: Detection and most probable number technique using 5-bromo-4-chloro-3-indolyl-ß-D-glucuronideInternational Standard · Published 18 May 2015 · ISO/TC 34/SC 9