Microbiology of food and animal feeding stuffs — Real-time polymerase chain reaction (PCR) for the detection of food-borne pathogens — General requirements and definitions

ISO 22119:2010 defines terms for the detection of food-borne pathogens in foodstuffs, and isolates obtained from them, using the polymerase chain reaction (PCR). ISO 22119:2010 specifies requirements for the amplification and detection of nucleic acid sequences (DNA or RNA after reverse transcription) by real-time PCR.

The minimum requirements laid down in ISO 22119:2010 provide the basis for comparable and reproducible results within individual and between different laboratories.

ISO 22119:2010 is also applicable, for example, to the detection of food-borne pathogens in environmental samples and in animal feeding stuffs.