ISO 23893-3:2013Published

Water quality — Biochemical and physiological measurements on fish — Part 3: Determination of vitellogenin

Qualité de l'eau — Mesurages biochimiques et physiologiques sur poisson — Partie 3: Dosage de la vitellogénine

Abstract / scope

ISO 23893-3:2013 specifies a method for measuring vitellogenin (Vtg) concentrations in a fish plasma sample employing an enzyme-linked immunosorbent assay (ELISA) method.

It applies to fish that are sampled in the environment (fresh, estuarine or salt water) and to fish exposed to substances or effluents in a laboratory. The method is quantitative when using Vtg antibodies and a Vtg standard well characterized with the species of choice.