Radiological protection — Performance criteria for laboratories using the cytokinesis block micronucleus (CBMN) assay in peripheral blood lymphocytes for biological dosimetry

ISO 17099:2014 addresses the following:

a) confidentiality of personal information for the customer and the laboratory;

b) laboratory safety requirements;

c) radiation sources, dose rates, and ranges used for establishing the calibration reference dose-effect curves allowing the dose estimation from CBMN assay yields and the minimum resolvable dose;

d) performance of blood collection, culturing, harvesting, and sample preparation for CBMN assay scoring;

e) scoring criteria;

f) conversion of micronucleus frequency in binucleated cells into an estimate of absorbed dose;

g) reporting of results;

h) quality assurance and quality control;

i) informative annexes containing examples of a questionnaire, instructions for customers, a microscope scoring data sheet, a sample report and advice on strengths and limitations of current automated systems for automated micronucleus scoring.