ISO 21149:2017Published

Cosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteria

Cosmétiques — Microbiologie — Dénombrement et détection des bactéries aérobies mésophiles

Abstract / scope

ISO 21149:2017 gives general guidelines for enumeration and detection of aerobic mesophilic bacteria present in cosmetics

- by counting the colonies on agar medium after aerobic incubation, or

- by checking the absence of bacterial growth after enrichment.

Because of the large variety of cosmetic products within this field of application, this method may not be appropriate for some products in every detail (e.g. certain water immiscible products). Other methods (e.g. automated) may be substituted for the tests presented here provided that their equivalence has been demonstrated or the method has been otherwise shown to be suitable.

If needed, microorganisms enumerated or detected may be identified using suitable identification tests described in the standards given in the Bibliography.

In order to ensure product quality and safety for consumers, it is advisable to perform an appropriate microbiological risk analysis to determine the types of cosmetic products to which this document is applicable. Products considered to present a low microbiological risk (see ISO 29621) include those with low water activity, hydro-alcoholic products, extreme pH values, etc.

Life cycle

Amendments & corrigenda

ISO 21149:2017/Amd 1:2022PublishedCosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteria — Amendment 1International Standard · Published 30 Aug 2022 · ISO/TC 217

Replaces

ISO 21149:2006WithdrawnCosmetics — Microbiology — Enumeration and detection of aerobic mesophilic bacteriaInternational Standard · Published 8 Mar 2006 · ISO/TC 217