ISO/TS 22939:2019Published

Soil quality — Measurement of enzyme activity patterns in soil samples using fluorogenic substrates in micro-well plates

Qualité du sol — Mesure en microplaques de l'activité enzymatique dans des échantillons de sol en utilisant des substrats fluorogènes

Abstract / scope

This document specifies a method for the measurement of several enzyme activities (arylsulfatase, α −glucosidase, β -glucosidase, Cellubisidase, β -Xylosidase, phosphodiesterase (PDE), chitinase, phosphomonoesterase (PME), leucine-aminopeptidase, Alanine-aminopeptidase) simultaneously (or not) using fluorigenic substrates in soil samples. Enzyme activities of soil vary seasonally and depend on the chemical, physical and biological characteristics of soil. Its application for the detection of harmful effects of toxic chemicals or other anthropogenic impacts depends on the simultaneous comparison of enzyme activities in a control soil similar to the test soil, or on exposure tests with chemicals or treatments.

Life cycle

Replaces

ISO/TS 22939:2010WithdrawnSoil quality — Measurement of enzyme activity patterns in soil samples using fluorogenic substrates in micro-well platesTechnical Specification · Published 8 Mar 2010 · ISO/TC 190/SC 4