ISO 16000-22:2025Published

Indoor air — Part 22: Detection and quantification of fungal biomass by fungal β-N-acetylhexosaminidase enzyme activity

Air intérieur — Partie 22: Détection et quantification de la biomasse fongique par caractérisation de l’activité de l'enzyme fongique β-N-acétyl-hexosaminidase

Abstract / scope

This document specifies requirements for the sampling and analysis of air, surface or bulk material samples analysed by fluorometric detection of an enzyme activity present in filamentous fungi[1] (US Patent No. 6,372,446) to quantitatively determine the total fungal biomass density. It describes the analytical procedure that can be performed on-site or in a laboratory. This method does not enumerate or differentiate genera or species of fungi.