ISO 17601:2025Published

Soil quality — Estimation of abundance of selected microbial gene sequences by quantitative polymerase chain reaction (qPCR) from DNA directly extracted from soil

Qualité du sol — Estimation de l’abondance de séquences de gènes microbiens par amplification par réaction de polymérisation en chaîne quantitative (qPCR) à partir d’ADN directement extrait du sol

Abstract / scope

This document specifies the crucial steps of a quantitative polymerase chain reaction (qPCR) method to measure the abundance of selected microbial gene sequences from soil DNA extract. The number of microbial gene sequences quantified by qPCR provides an estimation of the abundance of selected microbial groups in soil.

Life cycle

Replaces

ISO 17601:2016WithdrawnSoil quality — Estimation of abundance of selected microbial gene sequences by quantitative PCR from DNA directly extracted from soilInternational Standard · Published 12 Jan 2016 · ISO/TC 190/SC 4