Cigarettes — Determination of tobacco specific nitrosamines in the mainstream smoke with a smoking regime according to ISO 3308 (standard smoking regime)— Method using high performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS)

This document specifies a method for the quantification of four tobacco specific nitrosamines (TSNAs) in the total particulate matter of mainstream cigarette smoke by using reversed phase high performance liquid chromatography with tandem mass spectrometry (LC-MS/MS). The quantified TSNAs are: N-nitrosonornicotine (NNN), N-nitrosoanatabine (NAT), N-nitrosoanabasine (NAB) and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK).