ISO 18862:2025Published

Coffee and coffee products — Determination of acrylamide — Methods using high-performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS) and gas chromatography with mass spectrometric detection (GC-MS) after derivatization

Café et dérivés du café — Dosage de l’acrylamide — Méthodes par chromatographie liquide à haute performance avec détection par spectrométrie de masse en tandem (CLHP–SM/SM) et chromatographie en phase gazeuse avec détection par spectrométrie de masse (CG–SM) après dérivation

Abstract / scope

This document specifies methods for the determination of acrylamide in coffee and coffee products by extraction with water, clean-up by solid-phase extraction (SPE) and determination by high-performance liquid chromatography with tandem mass spectrometric detection (HPLC-MS/MS) and gas chromatography with mass spectrometric detection (GC-MS) after derivatization. The methods were validated in a validation study for roasted coffee, soluble coffee, coffee substitutes and coffee products with ranges from 53 μg/kg to 612,1 μg/kg.

Life cycle

Replaces

ISO 18862:2016WithdrawnCoffee and coffee products — Determination of acrylamide — Methods using HPLC-MS/MS and GC-MS after derivatizationInternational Standard · Published 11 Jul 2016 · ISO/TC 34/SC 15